Journal: Biosensors & bioelectronics
Article Title: Neuroadhesive protein coating improves the chronic performance of neuroelectronics in mouse brain
doi: 10.1016/j.bios.2020.112096
Figure Lengend Snippet: List of reagents used for this experiment.
Article Snippet: The reagents used in this experiment are shown in supplementary section , . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti- NeuN , Mouse Millipore Sigma MAB377 Anti-Neurofilament 200 kDa (NF- 200) Millipore MAB5256 Wako Chemicals USA Rabbit Ant- Iba1 , Rabbit Fisher NC9288364 Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein ( GFAP ) Dako Z033401 DyLight 594 labeled Lycopersicon Esculentum (Tomato) Lectin Vector Labs B1175 IgG Jackson ImmunoResearch Laboratories, Inc. 115–607-003 Fab Fragment Only Jackson ImmunoResearch Laboratories, Inc. 115–007-003 Alexa Fluor® 488 goat anti-mouse IgG Invitrogen A-11001 Alexa Fluor 488 Goat Anti-Rabbit IgG Invitrogen A-11034 Alexa Fluor® 568 goat anti-rabbit IgG Invitrogen A-11032 Alexa Flour 568 goat-anti mouse Invitrogen A-11004 Alexa Fluor® 633 goat anti-chicken IgG Invitrogen A-21103 Hoechst 33342, Trihydrochloride, Trihydrate Invitrogen H3570 Fluoromount-G Southern Biotech 0100–01 DyLight 649 Streptavidin Vector SA-5649 Chemicals Isothesia (isoflurane) solution Covetrus North America 029405 Experimental Models: Organism/Strain Mouse: C57BL/6J, male The Jackson Laboratory 000664 Other Recording electrode (1 shank, 16 channels NeuroNexus A1×x16–3mm-100- 703-CM16 Data acquisition system Tucker Davis Technologies RX7 Isoflurane Vaporizer Patterson Veterinary Inc. PAD, heat therapy SM 13” × 18” for HTP-1500 soft-temp localized heat therapy system by Adroit Medical Medline ADRST017H Stereotaxic Frame Kopf Instruments Burrs for Micro Drill Fine Science Tools, Inc. 19008–07 Self-T apping Bone Screws - 4.8mm/1.19mm Shaft Diameter Fine Science Tools, Inc. 327433–1.7G Kwik-Cast Sealant World Precision Instruments KWIK-CAST Triple Antibiotic Ointment Henry Schein 061148 Absorption Triangles Fine Science Tools, Inc 18105–03 Restoratives, Composite, Flowable - Natural Elegance Intro Kit Henry Schein 037752 Ketofen Ketoprofen 100mg Henry Schein 005487 Fisher Healthcare™ Tissue-Plus™ Fisher Scientific 23–730-571 O.C.T.
Techniques: Labeling, Plasmid Preparation, Ointment
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